About the Lectin

Crude extracts of mango seeds contain a non-specific hemagglutinin. As with the avocado lectin, MIA agglutinates several strains of Campylobacter jejuni and Campylobacter coli 1,2 , many different strains of Legionella 3 , and all strains of Brucella 4 . Agglutination of strains of Yersinia enterocolitica is correlated with the presence of the virulence plasmid. 6 MIA does react differently with certain micro-organisms than PAA. The mango lectin has been shown to agglutinate coagulase negative staphylococci, including S. epidermis, S. saprophyticus, and clinical strains of S. aureus 5 . MIA does not show blood group specificity, however, it does react better with type O and A1 cells than it does with either B or A2 cells. The carbohydrate specificity of MIA has not been determined. This lectin is only available in crude form.

REFERENCES

  1. Staat, R. H., et al. (1978) Adv. Exp. Med. Biol. 107 : 639-647.
  2. Corbel, M.J. and Gill, KP (1987) Vet. Microbiol. 15 : 163-173.
  3. Doyle, R. J., et al. (1982) J. Clin. Micro. 15 : 973-975.
  4. Corbel, M.J., et al. (1984) Devel. Biol. Standardization 56 : 169-175.
  5. Davidson, S. K., et al. (1982) J. Clin. Micro. 15 : 547-553.
  6. Wauters, G., et al. (1995) J. Clin. Microbial. 33 : 772-774.

Product Characteristics
Buffer 0.01M Phosphate – 0.15M NaCl, pH 7.2-7.4.
Blood Group Non-specific. O, A1 > B, A2.
Activity 20 mg/ml of crude lectin will agglutinate type B and A2 erythrocytes. 5 mg/ml will agglutinate type O and A1 cells. Less than 2.5 mg/ml will agglutinate neuraminidase treated erythrocytes.
Inhibitory Carbohydrate Not determined.